In this experiment processing coloured potatoes were selected as a testing materials．To study the effect on differenence medium of vigorous seedings of virus-free coloured potatoes plants inside test tube were in orthgonal design thogonal experiments of four factors and three levels．In order to select the best medium of vigorous seedlings of virus-free coloured potatoes plants inside test tube some elements were adjusted and made variance analysis based on the seedling height stem thick the number of stems and the number of roots after cultivate40days．The result showed that the best medium for growth of synthesized of virus-free coloured potatoes plants of inside test tube was MS+6-BA0．25mg/L＋IBA0．01mg/L＋sugar50g/L．