PDF(482 KB)
砷对3T3-L1前脂肪细胞增殖和分化的影响
张冰荫, 慕晓玲, 王瑞, 杨元元, 李媛媛, 李莉
PDF(482 KB)
PDF(482 KB)
砷对3T3-L1前脂肪细胞增殖和分化的影响
Effects of Arsenic on Proliferation and Differentiation of 3T3-L1 Preadipocytes
ZHANG Bingyin, MU Xiaoling, WANG Rui, YANG Yuanyuan, LI Yuanyuan, LI Li
To observe the effect of arsenic on the proliferation and differentiation of 3T3-L1preadipocytes. The differentiation of 3T3-L1preadipocytes was identified with red O staining, the proliferation of 3T3-L1 preadipocytes was detected by MT T and cell calculation.Lipid droplet s accumulated in cytoplasm of the differentiated preadipocytes were observed by using red O staining and quantified by colorimetry.The result s showed that the low concentration of arsenic (low than 5μmol/L) affected 3T3-L1 preadipocytes proliferation was significantly increased, but in contrast arsenic at different concentrations (5, 10, 15, 20 and 25μmol/L) which affected cell proliferation was decreased ( P<0.05, compared with the normal control group). Whatever arsenic at any concentration was significantly decreased the differentiation of adipocytes and does-dependently.Arsenic at low concentration can increase the proliferation of 3T3-L1 preadipocytes, and arsenic at high or low concentration inhibit the differentiation of adipocytes.
arsenic
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