为探讨在兔膝关节腔注射尿激酶型纤溶酶原激活物(urokinase-type plasminogen activator,uPA),建立兔膝关节骨关节炎动物模型的可行性。选择健康新西兰大白兔42只并随机分为实验组和对照组,实验组24只,对照组18只;实验组在右膝关节内注射uPA0.4μg/0.2mL,对照组注射等容量的生理盐水,注射完成后在4周、8周、12周分批取兔滑膜进行组织学观察、取软骨行组织学观察及电镜检查。结果显示:实验组兔膝关节滑膜均存在不同程度增生、肥厚、水肿,光镜及电镜下见实验组关节软骨呈明显退行性变。Mankin’s评分实验组得分显著高于对照组(P<0.05)。结论:兔膝关节注射uPA可使滑膜发生炎症、关节软骨发生退变,而且随着时间退变的程度逐渐加重,这一模型可为研究膝关节OA的病因、发病机理及治疗方法提供实验工具。

The aim of this study was to set up an animal model of osteoarthritis(OA) in the rabbit joint.Methods 42 white rabbits were devided into experimental animals and control animals.The experimental animals were injected intraarticularly with 0.4μg/0.2mL uPA,and control animals were injected with the same doses of normal saline.All animals were sacrificed 4,8,12 weeks respectively after the injections.The synovial tissue and the articular cartilage were pathologically examined.Results:The synovium of knee joint in the experimental group were found adhensive,swelling and overgrowth.Obvious degenerative change were found in the experimental group by microscope.The experimental group score was significantly higher than that of the compared group according to Mankin's stantard(P<0.05).Conclusion uPA may be responsible for the inflammation of synovium and degeneration of articular cartilage,and the degree of the degeneration was gradually aggravated by the extension of time.This animal model can be used for exploring pathogenisis of osteoarthritis and selecting treatments for this disease.