PDF(318 KB)
高效液相色谱法分析红色毛癣菌麦角甾醇的含量
李治建, 古力娜·达吾提, 斯拉甫.艾白, 安惠霞, 肖威
PDF(318 KB)
PDF(318 KB)
Determination of Ergosterol in Mycelium of Trichophyton Rubrum by High Performance Liquid Chromatography
LI Zh-i jian1, Gulina·Dawuti2, Silafu·Aibai2, 3, AN Hu-i xia1, XIAO Wei1
A determination method of ergosterol in mycelium of Trichophyton rubrum by reversed phase high performance liquid chromatography was established.The sample was analyzed after saponifing and extracting. The chromatographic column waters symmetry shieldTM RP C18(4.6mm×150mm, 5μm) was used with 100% methanol as the mobile phase. The flow rate was 1mL/min and the detected wavelength was set at 282nm. The analyte was qualitated by retention time and quantitated by peak area by using external standard method. The result shows that the retention time of ergosterol is 8.9min.There is excellent linearity between peak area and concentration of analyte in the concentration range of 0.93~29.75mg/L for ergosterol. The correlation coefficient is 0.9998. The average recoveries are94.8%~96.3%.The relative standard deviations are1.8%~3.3%. The detection limit of concentration is0.008mg/L.This method is accurate, simple and precise.
high performance liquid chromatography
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