PDF(733 KB)
牛松质骨脱细胞骨细胞外基质的制备
张永刚, 王德超, 石辉, 孙建华, 史晨辉, 董金波, 刘维钢,
PDF(733 KB)
PDF(733 KB)
牛松质骨脱细胞骨细胞外基质的制备
Preparation of Acellular Bone Extracellular Matrix of Bovince Cancellous Bone
ZHANG Yong-gang, WANG De-chao, SHI Hui, SUN Jian-hua, SHI Chen-hui DONG Jing-bo, LIU We-igang
The paper is to find a new kind of bone substitute-acellular bone extracellular matrix (ABECM) available for bone implant and find the best methods of preparation of acellular bovince cancellous bone.Mathods:bovince upper humerus cancellous bone was approached acellularly by using0.5%sodium chloride, 10% sodium chloride and TritonX100according to the analytic factorial experiment.Specimens were stained with HE, Massion and observed with naked eyes, microscopy and scanning electron microscopy, to find the best mathod of ABECM.Results showed there were no cellular components in respectively histological slides of the bones, but the basic structure of extracellular matrix existed in 10% sodium chloride, 0.5%TritonX-100in48hrs.These indicate that ABECM can be obtained successfully with this procedure, TritonX-100is a good reagent for preparing ABECM.ABECM may be a new kind of ideal boned substitute and will be applied widely in the future.
/ "> bone / acellular bone extracellular / bone substitute / tissue engineering {{custom_keyword}} /
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