绵羊防御素sBD-1 cDNA的克隆及序列分析

盛金良, 王金富, 胡文革, 魏虹

PDF(213 KB)
PDF(213 KB)
石河子大学学报 ›› 2003, Vol. 21 ›› Issue (1) : 1-3.

绵羊防御素sBD-1 cDNA的克隆及序列分析

  •   盛金良,王金富,胡文革,魏虹
作者信息 +

The cDNA Cloning and Ceqencing of Cefensin from Ovine Tongue

  • SHENG Jin-liang1, WANG Jin-fu2, HU Wen-ge1, Wei Hong2

Author information +
History +

摘要

从成年绵羊舌表皮组织分离提取总RNA,经反转录PCR(RT PCR)扩增出绵羊防御素sBD 1cDNA,将扩增片段回收,重组插入克隆载体T vector,经限制性酶切鉴定和DNA序列测定,结果扩增出cDNA215bp,与发表的绵羊sBD 1cDNA序列完全相同。该cDNA编码64个氨基酸,其中信号肽与前片段相同,成熟肽位于前片段的羧基端,由38个氨基酸残基组成。

Abstract

Total RNA were isolated from adult ovine tongue ti ss ue. The ovine β defensin 1(sBD1)cDNA was amplified by the reverse transcript ion polymerase chain reaction (RTPCR). The cDNA was inserted into cloning vect or (Tvector).And the cDNA was identified by restriction endonuclease and sequ enced it.The cDNA has the same sequence sBD1 cDNA as reported,this cDNA len gth is 215 bp and encode 64 amino acid,including sequence encoding propiece and mature peptide.

关键词

/ uniplatform=NZKPT& / language=CHS">防御素 / / uniplatform=NZKPT& / language=CHS">逆转录聚合酶链式反应 / / uniplatform=NZKPT& / language=CHS">克隆 /

Key words

defensin / reverse transcription polymerase chain rea ction / clone

引用本文

导出引用
盛金良, 王金富, 胡文革, 魏虹. 绵羊防御素sBD-1 cDNA的克隆及序列分析. 石河子大学学报. 2003, 21(1): 1-3
SHENG Jin-liang, WANG Jin-fu, HU Wen-ge, Wei Hong.

The cDNA Cloning and Ceqencing of Cefensin from Ovine Tongue

. Journal of Shihezi University. 2003, 21(1): 1-3

参考文献

[1] 陈 颖, 葛毅强, 张利明, .哺乳动物防御素的研究进 展及其应用前景.[J] 生物化学与生物物理进展, 2001, 28(1):17-21.

[2] Nicolas P, Mor A.Peptide as weapons against microorganisms in the defense system of vertbrates[J].Annu Rev Microbiol, 1995, 49:277-280.

[3] Kenneth M, Huttner, Donna J, et al, Antimicrobial peptide expression is developmentally regulated in the ovine gastrointestinal tract[J].JNutr, 1998, 128:297s-299s.

[4] Bomam H G.Antimicrobial Peptides [M].New York:Wiley, 1994, 250-269.

[5] Gill Diamond, Michael Zasloff, Howard Eck, et al.Tracheal antimicrobial peptide, a cysteine-rich peptide from mammalian tracheal mucosa:Peptide isolation and cloning of a cDNA[J].Proc Natl Acad Sci, 1991, 88:3952-3956.

[6] Piers K L, Brown M H, Hancock R E W.Recombinant DNA procedures for producting small antimicrobial cationic peptides inbacterial[J].Gene, 1993, 134:7-13.

PDF(213 KB)

5

Accesses

0

Citation

Detail

段落导航
相关文章

/