目的 探讨基质金属蛋白酶-3(matrix metalloproteinase 3,MMP-3)-1171 5A/6A基因多态性与新疆地区汉族、维吾尔族人群膝骨性关节炎(primary knee osteoarthritis,PKOA)的关联。方法 通过病例-对照研究的方法,选取新疆部分地区汉族OA患者111例,NOA健康体检对象217例,维族OA患者105例,NOA对象201例,利用限制性片段长度多态性聚合酶链反应(PCR-RFLP)对所选对象MMP-3-1171 5A/6A基因多态性进行检测并行相关性分析。结果 汉族及维吾尔族 MMP-3-11715A/6A基因型分布及等位基因频率符合 Hardy-Weinberg遗传平衡(P ＞ 0.05),MMP-3-1171 5A/6A基因型和等位基因频率在汉族与维族OA人群中分布差异有统计学意义(χ2=11.206和8.526,均P值＜0.01),维吾尔族人群中MMP-3-1171 5A/6A等位基因频率在OA组及NOA组之间差异有统计学意义(χ2=10.648,P＜0.01),其中5A等位基因相较于6A等位基因可以提高维族OA的患病风险(OR=1.793,95% CI:1.270～2.532)。结论 MMP-3-1171 5A/6A 基因多态性与维族人群OA相关,5A等位基因可能增加维吾尔族骨性关节炎的患病风险。
Abstract
Objective The aim to investigate the revelance of MMP-3-1171 5A/6A gene polymorphism on primary knee osteoarthritis in Han and Uygur populations in parts of xinjiang. Methods In a case-control study,111Han patients with OA,217patients with NOA,105Uygur patients with OA and 201patients with NOA were selected in some areas of Xinjiang.The MMP-3-1171 5A/6A gene polymorphism of the selected subjects was detected by PCR-RFLP,and the correlation analysis was carried out. Results The distribution of MMP-3-1171 5A/6A genotypes and allele frequencies in Han and Uygur nationalities were consistent with Hardy-Weinberg genetic equilibrium (P>0.05).The frequency of MMP-3-1171 5A/6A genotype and allele gene of Han was significantly different from Uighur in osteoarthritis(OA)(χ2=11.206 and 8.526,P＜0.01).The frequency of MMP-3-1171 5A/6A genotype of Uighur had significantly difference between OA and NOA (χ2=10.648,P＜0.01),and the allele gene of 5A may significantly Improve the risk of OA (OR=1.793,95% CI:1.270～2.532). Conclusion The SNPs of MMP-3-1171 5A/6A is probably associated with osteoarthritis in Uygur nationality,and 5A allele may increase the risk of osteoarthritis in Uygur nationality.
关键词
MMP-3 /
基因多态性 /
骨关节炎
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Key words
MMP-3 /
gene polymorphism /
osteoarthritis
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脚注
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基金
国家自然科学基金项目(81660374)
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