为了探讨猪 FOXO3 基因 SNPs 位点及其与猪生长性状的关联性，采用基因克隆并测序的方法检测 FOXO3 基因的
多态位点，并对其与生长性状进行关联分析。结果显示：该基因共存在 3 个 SNP 位点，为：G82316A、T60086C 和
A64011G，分别命名为：P1、P2 和 P3。在群体中均表现出 3 种基因型，3 个位点对生长性状的影响表现为：P1 位点 AA 基
因型个体校正背膘厚显著高于 AG 基因型个体（P<0.05）以及 GG 基因型个体（P<0.05）；P2 位点 CC 基因型个体背膘厚
显著高于 TT 基因型个体（P<0.05）以及 TC 基因型个体（P<0.05）；P3 位点 AG 基因型个体 100 kg 日龄显著低于 AA 基
因型个体（P<0.05）以及 GG 基因型个体（P<0.05）。结论：P1、P2 变异位点对大白猪的背膘厚及生长速度具有负选择作
用，P3 位点对大白猪的背膘厚和生长速度具有正选择作用。此 3 个多态位点可作为猪分子遗传育种的选择性标记。
Abstract
To investigate SNPs in FOXO3 gene and the correlation between the SNPs and growth traits of pigs. Gene cloning
and sequencing were adopted to investigate the SNPs, and its association with growth traits were analyzed. The result showed
that a total of three SNPs were detected in FOXO3 gene, such as G82316A, T60086C and A64011G，they were respectively
named P1, P2, P3. The three sites were all expressed as three genotypes. The influence of the three sites on growth traits
showed that the corrected back fat thickness and the age of reaching 100kg of pigs with AA genotype were significantly higher
than the ones with AG genotype(P<0.05) and the ones with GG genotype (P<0.05) in P1; the back fat thickness and the age of
reaching 100kg of pigs with CC genotype were significantly higher than the ones with TT genotype (P<0.05) and the ones with
TC genotype (P<0.05) in P2；the back fat thickness and the age of reaching 100kg of pig with AG genotype was significantly
lower than the ones with AA genotype (P<0.05) and GG genotype (P<0.05) in P3. P1 and P2 sites display negative selection
effects on back fat thickness and growth rate; P3 site displays positive selection affact the back fat thickness and growth rate.
Therefore the sites can be selected as assistant-selected genetic markers for pig breeding.
关键词
猪 /
FOXO3 基因 /
多态性 /
生长性状 /
关联分析
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Key words
pig /
FOXO3 gene /
polymorphism /
growth traits /
correlation analysis
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脚注
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基金
国家重大科学计划项目（2014CB138504），国家自然科学基金项目（31171192）
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