氮掺杂多孔碳具有良好的生物相容性，聚邻苯二胺具有丰富的氨基以及亚氨基，能提高电化学免疫传感器的
性 能。本 文 构建 了 一 种 具 有 较 高 选 择 性 和 稳 定 性 以 及 灵 敏 性 的 无 标 记 型 电 化 学 免 疫 传 感 器 用 于 检 测 甲 胎 蛋 白
（AFP）。基于氮参杂多孔碳 / 聚邻苯二胺修饰电极表面，采用循环伏安（CV）及交流阻抗法（EIS）研究了修饰电极表
面的电化学特性。在优化的条件下，该电化学免疫传感器的相应电流与 AFP 的浓度在 0.005- 100 ng/mL 范围内呈现
良好的线性关系，检测限为 0.0006 ng/mL。新型电化学免疫传感器可为临床应用提供潜在的应用。
Abstract
In this study, we constructed a label- free electrochemical immunosensor for highly selective detection， stability and
sensitive detection of alpha- fetoprotein (AFP) based on PoPD and N- doped porous carbon. The electrochemical characteristics
with the modified electrode surface were studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS)
measurements. The using of N- doped porous carbon greatly enhanced the biology catalytic activity due to its large specific
surface and excellent biocompatibility. PoPD has large amino which can improved its ability to fixed anti- AFP. Under the
optimized conditions, the results show that the immunosensor have excellent electrochemical properties with a wide concentration
range from 0.005 to 100 ng/mL and a detection limit of 0.0006 ng/mL. The new type of electrochemical immunosensor may
provide potential applications for the clinic application.
关键词
聚邻苯二胺 /
多孔碳 /
氮掺杂 /
甲胎蛋白 /
免疫传感器
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Key words
POPD /
prous carbon /
N- doped /
alpha- fetoprotein /
immunosensor
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参考文献
[1] Wang H,He L,Zhang Y,et al.Label- free immunosensor based on Pd nanoplates for amperometric immunoassay of
alpha- fetoprotein [J].Biosensors & Bioelectronics,2013,53C
(6):305- 309.
[2] Zhou H,Gan N,Li T,et al.The sandwich- type electrochemiluminescence immunosensor for α- fetoprotein based on
enrichment by Fe3O4- Au magnetic nano probes and signal amplification by CdS- Au composite nanoparticles labeled anti- AFP[J].Analytica chimica acta, 2012,746:107- 113.
[3] 王知.甲胎蛋白生物传感器的制备和研究[D].苏州：苏州大
学,2009.
[4] And E C,C J C.A Novel Self- Assembled Nanoparticulate
Film for Covalent Attachment of Antibodies to Plastic [J].
Langmuir,2003,19(10):4509- 4511.
[5] Lin J,Ju H.Electrochemical and chemiluminescent immunosensors for tumor markers [J].Biosensors & Bioelectronics,
2005,20(8):1461- 1470.
[6] Bahadr E B,Sezgintürk M K.Applications of electrochemical immunosensors for early clinical diagnostics[J].Talanta,
2015,132:162- 174.
[7] Loomans E E M G,Roelen A J M,Van Damme H S,et al.
Assessment of the functional affinity constant of monoclonal antibodies using an improved enzyme- linked immunosorbent assay[J].Journal of Immunological Methods,1995,184
(2):207- 217.
[8] Li Q,Zeng L,Wang J,et al.Magnetic Mesoporous Organic
Inorganic NiCo2O4 Hybrid Nanomaterials for Electrochemical Immunosensors[J].Acs Appl mater interfaces,2011,3(4):
1366- 1373.
[9] Kroto H W,Heath J R,O'Brien S C,et al. C60:Buckminsterfullerene[J].Nature,1985,318(6042):162- 163.
[10] Orikasa H,Akahane T,Okada M,Tong Y,Ozaki J- i,Kyotani
T.Electrochemical Behavior of Carbon Nanorod Arrays Having Different Graphene Orientations and Crystallinity[J].J
Mater Chem,2009,19(26):4615- 4621.
[11] Sun X,Li Y.Colloidal Carbon Spheres and Their Core/Shell
Structures with Noble- Metal Nanoparticles [J].Angew Chem
Int Ed,2004,43:597- 601.
[12] 黄维垣.高技术有机高分子材料进展[M].北京：化学工业
出版社, 1994.
[13] Qiu J D,Huang H,Liang R P.Biocompatible and label- free
amperometric immunosensor for hepatitis B surface antigen
using a sensing film composed of poly(allylamine)- branched ferrocene and gold nanoparticles[J]. Microchimica Acta,
2011,174(1- 2):97- 105.
[14] Xiao S,Zheng C,Liang L,et al.One- Pot Synthesis and Catalyst Support Application of Mesoporous N- Doped Carbonaceous Materias[J].J Mater Chem,2012,22(24):12149- 12154.
[15] Zhen Y L,Guo X Z,Zhi Y L,et al.One- Step Scalable Preparation of N- doped Nanoporous Carbon as High- Performance Electrocatalysts for Oxygen Reduction Reaction [J].
Nano Res,2013,6(4):293- 301.
[16] Ghalkhani M,Shahrokhian S.Application of carbon nanoparticle/chitosan modified electrode for the square- wave adsorptive anodic striping voltammetric determination of Niclosamide[J].Electrochemistry Communications,2010,12(1):66-
69.
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脚注
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基金
教育部科学技术研究重点项目（21065009）
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