The changes of the regulator y T cell level and the expression of connexin
40 in the perimenopause Rats' peripheral blood
Author information+
(1 Department of Physiology, School of Medicine Shihezi University/Laboratory for Xinjiang Endemic and Ethnic Diseases/ School
of Medicine, Shihezi University, Shihezi, Xinjiang 832002, China; 2 Department of Obstetrics and Gynecology, the First
Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang 832002, China)
To observe peripheral blood expression differences of T lumphocyte subsets and Connexin 40 (Cx40) in normal and
perimenopause Sprague- Dawley (SD) rats and to investigate the relationship between these differences and immunoregulation.18
four- month female SD rats were evenly divided into the control group and the OVX group (ovariectomized group). In the latter
group, a perimeopause model was established four weeks after subjects' bilateral ovaries were exercised. Then flow cytometry was
applied to detect T lymphocyte subsets of peripheral blood and the expression of Cx40 in T lymphocyte subsets in each group
respectively. Meanwhile, ELISA method was used to detect secretion of such inflammatory factors IL- 1, IL- 2, IL- 6 in each group.
The result shows that the CD3+ and CD4+ lymphocytes in peripheral blood of OVX group decreased by 13.24% (P<0.05) and
22.5% (P<0.05) when compared with control group respectively; while that of CD8+ increased by 29.8% (P<0.05) and that of
CD4+/CD8+ decreased (P<0.01). The results also show that the expression of Cx40 in CD4+ lymphocytes in OVX group was 6.54±
1.75% which was higher than that of control group (4.01±2.51%) (P<0.05). The expression of Cx40 in the CD8+ lymphocytes of theOVX group was 18.3%±5.53% which was obviously higher than that of control group (8.05%±4.17%) (P<0.01). Compared with
control group, in OVX group there was no statistical significance in the difference of IL- 1β level (P>0.05), while the level of IL- 2
decreased (P<0.05) and that of IL- 6 increased (P<0.01). It can be concluded that for perimenopause rats the ratio of lymphocyte
subsets CD4+ and CD8+ decreased while expression of Cx40 in the CD4+ and CD8+ lymphocytes of peripheral blood increased,
which indicates that the level of estrogen is closely related to communicating junction protein expression in lymphocytes and the
level also might be involved with regulation of immune response process in the perimenopause period.
Fu Ruiting, Peng Min , Si Junqiang , Ma Ketao , Li Li.
The changes of the regulator y T cell level and the expression of connexin
40 in the perimenopause Rats' peripheral blood. Journal of Shihezi University. 2017, 35(6): 726-731 https://doi.org/10.13880/j.cnki.65- 1174/n.2017.06.011
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