四种土壤处理除草剂对棉花叶片氧化应激防御系统 及根系活力的影响

李亚东, 梁友, 韩小强, 杨德松

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石河子大学学报 ›› 2016, Vol. 34 ›› Issue (6) : 703-708. DOI: 10.13880/j.cnki.65- 1174/n.2016.06.008
农学·植物保护

四种土壤处理除草剂对棉花叶片氧化应激防御系统 及根系活力的影响

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Effects of four kinds of soil- applied her bicides on oxidative stress defense system and r oot activity of cotton

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摘要

为了棉田除草剂的科学合理的使用提供科学依据,本研究采用盆栽法对不同浓度乙氧氟草醚、五氟磺草胺、二甲 戊灵、仲丁灵对棉苗抗坏血酸含量、抗氧化酶活性、膜脂过氧化、根系活力等生理指标的影响进行了研究。结果显示:施 药 30 d 后,120、180、240 g/hm2 乙氧氟草醚,619、742 g/hm2 二甲戊灵,1008、1152 g/hm2 仲丁灵对棉花幼苗活性氧的积 累、细胞膜过氧化、根系活力无显著影响;360 g/hm2 的乙氧氟草醚、1113 g/hm2 的二甲戊灵、1584 g/hm2 的仲丁灵则显 著减少活性氧的积累、明显抑制棉花幼苗根系活力和破坏细胞膜完整性;五氟磺草胺各处理均显著影响棉花的氧化应 激防御系统,造成活性氧的累积,细胞膜完整性的破坏和根系活力的降低。结论:对棉花较为安全的乙氧氟草醚临界浓 度为 240 g/hm2 ,二甲戊灵临界浓度为 742 g/hm2 ,仲丁灵临界浓度为 1152 g/hm2 ,超出临界浓度会不同程度抑制棉花幼 苗的生长;五氟磺草胺各处理浓度对棉花生长影响较为严重,棉田杂草防除中不建议使用。

Abstract

In order to provide scientific basis for the rational use of herbicides in cotton fields, in this study we analyzed the content of ascorbic acid, antioxidant enzyme activity, lipid peroxidation, and root activity of cotton after treated with different concentrations of oxyfluorfen, penoxsulam, pendimethalin, butralin by pot- culture method. The results showed that the accumulation of active oxygen, cell membrane peroxiadation, and root vigor were not significantly affected after application of 120, 180 and 240 g/hm2 oxyfluorfen, 619 and 742 g/hm2 pendimethalin, 1008 and 1152 g/hm2 butralin for 30 days. While 360 g/hm2 oxyfluorfen, 1113 g/hm2 pendimethalin and 1584 g/hm2 butralin significantly inhibited the root activity, damaged the cell membrane and decreased the accumulation of active oxygen of cotton plants. All treatments of penoxsulam had a significant impact on cotton oxidative stress defense system, which resulted in the accumulation of active oxygen, decrease of root activity and the destruction of cell membrane integrity. It was concluded that 240 g/hm2 oxyfluorfen, 742 g/hm2 pendimethalin, 1152 g/hm2 butralin were the critical safe concentrations to cotton, exceedingr the critical concentrations will inhibit the growth of cotton seedling, the different concentrations of penoxsulam on the growth effect of cotton were more serious, and it is not recommended for weed control in cotton field.

关键词

土壤处理除草剂 / 棉花 / 氧化应激防御系统 / 根系活力 

Key words

soil- applied herbicides / cotton / oxidative stress defense system / root activity

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李亚东, 梁友, 韩小强, 杨德松 . 四种土壤处理除草剂对棉花叶片氧化应激防御系统 及根系活力的影响. 石河子大学学报. 2016, 34(6): 703-708 https://doi.org/10.13880/j.cnki.65- 1174/n.2016.06.008
LI Yadong, LIANG You, HAN Xiaoqiang, YANG Desong. Effects of four kinds of soil- applied her bicides on oxidative stress defense system and r oot activity of cotton. Journal of Shihezi University. 2016, 34(6): 703-708 https://doi.org/10.13880/j.cnki.65- 1174/n.2016.06.008

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基金

国家高技术研究发展计划(863 计划)项目(2011AA100508)
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