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小鼠肝细粒棘球蚴感染模型中 TLＲ2 的 表达及其对肝组织的影响
刘坪, 董丹, 王二强, 徐小丹, 孟娟娟, 侯隽, 王仙, 吴向未, 陈雪玲
PDF(1274 KB)
PDF(1274 KB)
小鼠肝细粒棘球蚴感染模型中 TLＲ2 的 表达及其对肝组织的影响
Expression of TLＲ2 in mouse liver Echinococcus granulosus infection model and its effect on liver tissue
Objective To investigate the expression of TLR2 in mouse liver echinococcus granulosus infection model and its effect on liver tissue.Methods Forty C57BL/6 mice were randomly divided into a control group and an infected group.The infected group was injected with 0. 1 mL of protoscolices suspension ( including 5000 protoscolices) under the liver capsule to establish secondary infection of Echinococcus granulosus.The model and the control group were injected with the same amount of PBS.The mice were sacrificed on days 3,7,14,and 21 after infection,and the livers were harvested to observe the general morphology of the livers,followed by HE staining, qRT-PCR,and immunohistochemistry to detect TLR2 expression in the liver.Results Compared with the control group,the mice in the model group were generally in good condition with an infection rate of 100%.With the prolonged infection time,the cysts on the surface of the liver tissue increased and protruded from the surface of the liver.HE staining showed inflammatory cell infiltration in liver lesion and fibrous tissue hyperplasia and other pathological changes.The relative expression of TLR2 mRNA and protein in the model group increased significantly,and as the infection time prolonged,the positive expression continued to be high.Conclusion After Echinococcus granulosus infection of the host,the expression of TLR2 in liver tissue increased,and obvious inflammation changes appeared.
细粒棘球蚴 / 肝脏 / Toll 样受体 2 {{custom_keyword}} /
Echinococcus granulosus / liver / Toll-like receptor 2 {{custom_keyword}} /
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