
棉花黄萎病菌细胞壁几丁质和壳聚糖构成分析
张博森, 赵汗青, 高峰
棉花黄萎病菌细胞壁几丁质和壳聚糖构成分析
Analysis the chitin and chitosan composition in Verticillium dahliae cell wall
Chitin and chitosan are important components of the fungal cell wall and play important roles in maintaining cell stability and host interaction. To analyze the composition of chitin and chitosan in the cell wall of Verticillium dahliae Kleb at different developmental stages, the strong pathogenic strain V592 was employed to observing the composition of chitin and chitosan in the cell wall of Verticillium dahliae at different developmental stages through microscope with CFW staining and chitosan antibody labeling technology. The results showed that the composition of chitin and chitosan in the cell wall of V. dahliae was varied in a dynamic process. Except for the cell wall of the conidial germination point, there is chitin in the cell wall at all other stages. Meanwhile, chitosan is mainly present at the location of the conidial germination point and the cell wall of the germ tube, and chitosan is also foundon the cell wall of the infected structure. This study provides a foundation for understanding the developmental biology of V. dahliae cell wall.
大丽轮枝菌 / 细胞壁 / 几丁质 / 壳聚糖 {{custom_keyword}} /
Verticillium dahlia / cell wall / chitin / chitosan {{custom_keyword}} /
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